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KMID : 0380219940270060522
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Journal of Biochemistry and Molecular Biology
1994 Volume.27 No. 6 p.522 ~ p.525
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Bioluminescent Assay of Phospholipase A2 Using Luminescent Marine Bacterium Vibrio harveyi mutant M-17
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Abstract
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Abstract:
@EN A new bioluminescent assay method for the activity of phospholipase A2(PLA2; phosphatitylcholine 2-acylhydrolase: EC 3.1.1.4) was developed using bioluminescent marine bacteria. A dark mutant of Vibrio harueyi (designated M-17), the in vivo
light
intensity of which was stimulated up to one thousand fold in the presence of myristic acid, was used for this assay. Sn-1-Stearoyl-sn-2myristoryl choline and bovine pancreas phospholipase A2 were used in this assay system, and produced myristic
acid was
quantified using V. harveyi mutant M-17. Activity was detected in less than one min with as little as 5 mU of phospholipase A2 and 5 nM of myristic acid production.
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KEYWORD
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